WebThe protocol we report here is based on biolistic delivery of transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin or kanamycin resistance and regeneration of plants with uniformly transformed plastid genomes. Because the plastid genome of Nicotiana tabacum derives from Nicotiana sylvestris, and the two ... WebNov 21, 2016 · This study reported the complete nucleotide sequence of the Nicotiana tabacum TN90 chloroplast (cp) genome. The cpDNA was 155 992 bp in length and …
Chloroplast proteome analysis of Nicotiana tabacum …
WebSummaryNicotiana tabacum chloroplast DNA was digested with several restriction endonucleases chosen for their potential usefulness in distinguishing between species of the genus, Nicotiana. The resulting fragments were ordered into a circular configuration of about 160 kilobase pairs, equalling about 100x106 daltons. WebNicotiana tabacum contains 0.5–9% nicotine, which is the primary toxin. Nicotine binds to select acetylcholine receptors throughout the body; known as nicotine receptors. This produces initial stimulation, but inhibition later, at the … song street life from jackie brown
Physical mapping of Nicotiana tabacum chloroplast DNA
Web6 hours ago · Leaf senescence reduces the photosynthetic capacity of leaves, thus significantly affecting the growth, development, and yield formation of cotton. Melatonin (MT) is a multipotent substance proven to delay leaf senescence. However, its potential mechanism in delaying leaf senescence induced by abiotic stress remains unclear. This … WebChloroplast type (N. tabacum = NT or N. knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloroplast DNA. Regenerates contained NT (13 plants) or NK (15 plants) plastids but only the plants with the NT chloroplasts were resistant to streptomycin. WebSep 15, 1997 · Fine mapping of replication origins (ori A and ori B) in Nicotiana tabacum chloroplast DNA Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B … songstress dnd 5e mystic melody